转基因耐旱、抗盐棉花新品种培育研究
The breeding of new cultivars of transgenic cotton with drought and salt resistance
  • 基本信息
  • 报告类型:  最终报告 编制日期:  2021-03-08 公开范围:  公开 全文页数:  30
    中文关键词:  转基因;耐旱;抗盐;新品种;棉花
    英文关键词:  transgenic;drought resistance;salt resistance;new cultivars;cotton
  • 中文摘要

本研究以现有转Bt基因抗虫棉材料和抗旱耐盐的非转基因材料,选育适于盐碱地、旱地、瘠薄地种植的抗旱、耐盐碱的高产、抗病、优质、抗衰的新品种或杂交种2个,并制定了相应的栽培技术;筛选耐旱、耐盐碱棉花新品系5份;鉴定出符合考核指标的转AhCMO和转AhBADH的棉花新种质9份,在山东棉花中心临清试验站考察了系选自28个转CMO和2个转BADH的转基因line的48个株行材料的产量和品质性状,共有来源于12个(转BADH的2个,转CMO的10个)独立转基因事件的13个株系的皮棉产量超过目前大面积推广的对照品种鲁棉研28号的90%,其中10个株系的皮棉产量高于对照;克隆了与植物耐逆性相关的2个基因,构建了适用于转基因植物安全的以Bar基因为标记的植物表达载体及以自主克隆的已锂为筛选标记的植物表达载体,并构建了适用于转化后删除选择标记基因的双T载体。本研究筛选鉴定的耐旱、耐盐性较强的转基因抗虫棉品种和新品系、创造的转AhCMO、AhBADH的耐盐、耐旱新种质以及鉴定克隆了新的抗性基因(AhPHD1和NEK6)。这些工作为加快转基因抗盐、耐旱材料的育种改良进程,通过分子设计育种培育综合性状优良的转基因棉花新品种或杂交组合,满足盐碱地和旱地棉花生产的需要奠定了基础。

  • 英文摘要

Two varieties we breeded suitable for planting in saline-alkali land, dry land, barren land, planting of drought resistance of high yield, disease resistance, alkali, high quality by the existing transfer Bt gene cotton material and drought resistance and salt tolerance of non-gmo material and formulate the corresponding cultivation techniques; Five new germplasms were screened for drought resistance, alkali cotton; Nine cotton new germplasms including AhCMO and AhBADH resistant for saline were identified; Yield test were investigated in at the Experimental Station of Shandong Cotton Research Center (ESSCRC)in Linqing county, Shandong province, and lint yields of 13 germplasms more than 90% of LMY28, which is the current widespread promotion. The yield for ten of 13 germplasms were more than that of LMY28. Two genes associated with plant resistancewere cloned. The safety of applicable to transgenic plants to the Bar to the genes of plant expression vector and to independent cloning has lithium plant expression vector for selection markers, and build the applicable to delete selection marker gene into double T carrier. This study screening of drought resistance, salt resistance cotton varieties and new strains, creating new germplasm including AhCMO, AhBADH resistance to salt, drought, and cloning the new resistance gene (AhPHD1 and NEK6). These results would speed up the breeding of transgenic resistance to salt and drought tolerant material improvement process. Also this study would laid a foundation for meeting the needs of the saline-alkali land and dryland cotton production through molecular design breeding to cultivate excellent comprehensive properties of transgenic cotton varieties or hybrid combinations.